日韩视频在线观看亚洲-美日韩在线视频-国产精品久久蜜臀av-91沈先生极品嫩模在线观看-国产中文字幕在线网站-激情五月天春色激情-国产熟女福利一区二区在线视频-日韩av一区偷拍-av 狠狠干狠狠操,中文字幕精品熟女人妻第一区,国产欧美日韩另类在线专区,亚洲欧洲成人久久桃色一区二区

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑

Principle

Effectene Transfection Reagent is an innovative non-liposomal lipid formulation that is used in conjunction with a special DNA-condensing enhancer and optimized buffer to achieve high transfection efficiencies. The enhancer first condenses the DNA molecules and Effectene Reagent subsequently coats them with cationic lipids （see figure "Model of Effectene Principle"）, providing a particularly efficient way of transferring DNA into eukaryotic cells. Effectene Reagent offers significant advantages over many liposome reagents and other transfection methods （1, 2）.

Procedure

The Effectene procedure has two steps. DNA is first mixed with Enhancer and a buffer that provides optimal salt conditions for efficient DNA condensation. This step requires just 2–5 minutes. Effectene Reagent is then added and the mixture is incubated for 5–10 minutes to allow Effectene–DNA complexes to form. The complexes are mixed with growth medium （which can contain serum and antibiotics）, and added directly to the cells. The cells are then incubated until harvested and analyzed for gene expression （see flowchart "Effectene Transfection Reagent"）.

Applications

Effectene Reagent is suitable for transient and stable transfection of a broad range of cell types （see figures "High Transfection Efficiencies Using Effectene Reagent" and "Transfection of Oligonucleotides Using Effectene Reagent"）. Because transfection can be performed in the presence of serum and requires low amounts of DNA （see figure "Serum and DNA Quantity vs. Transfection Efficiency"）, cytotoxicity is minimal. This makes Effectene Reagent particularly effective for primary cells （see figure "Effectene Reagent with Primary Cells"）. A searchable list of cell lines and primary cells successfully transfected using Effectene Reagent, as well as customer-developed transfection protocols, is available at the Transfection Tools web site — www.qiagen.com/TransfectionTools. View cell lines successfully transfected with this product.

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑

High-throughput transfection

The application of recombinant DNA technology to fields such as drug discovery and development has led to an increased need for high-throughput transfection. Transfection using Effectene Reagent requires low amounts of DNA and minimal handling, and in addition, removal of transfection complexes is not required, making this reagent highly suitable for high-throughput screening. Effectene Reagent provides outstanding transfection efficiencies, excellent reproducibility, and minimal cytotoxicity in high-throughput transfection, and is available in bulk quantities.

Cited References

1. Ausubel, F.M., et al. eds. （1991） Current Protocols in Molecular Biology, Wiley Interscience, New York.
2. Sambrook, J., Fritsch, E.F., and Maniatis, T., eds. （1989） Molecular cloning — a laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

Images

Model of Effectene Principle High Transfection Efficiencies Using Effectene Reagent Transfection of Oligonucleotides Using Effectene Reagent Effectene Transfection Procedure Serum and DNA Quantity vs. Transfection Efficiency Effectene Reagent with Primary Cells

Effectene Transfection Reagent  effectene轉(zhuǎn)染試劑 詳細產(chǎn)品信息可和選購